Description /Descripción

Madrid Declaration on Ozone Therapy

ISCO3, 4th edition, 2025 – Digital English Version (view-only)

The Madrid Declaration on Ozone Therapy – For the Unification of Criteria in the Practice of Ozone Therapy is the only truly global reference document on ozone therapy.

The 4th edition (2025) is a comprehensively expanded and fully updated version that covers:

• Human Medicine

• Dentistry

• Veterinary Medicine (small and large animals)

It compiles scientific research from around the world and the accumulated clinical experience of ozone therapists from many different countries, fostering a global dialogue to improve clinical outcomes and scientific standards.

This product gives access to the 4th edition in English (digital format only).
For viewing only; it cannot be copied, printed, or downloaded.

Main features of the 4th edition (2025)

• Official ISCO3 document, approved on April 18, 2025

• Expanded and fully updated scientific content

• Includes addenda for Dentistry and Veterinary Medicine

• Clear therapeutic ranges, indications, contraindications and safety guidelines

• Practical orientation for daily clinical use

The Declaration serves as a reference and guide for healthcare professionals. The application of its recommendations always depends on the clinical judgment and responsibility of each health professional.

It is a living document, in force for 15 years and reviewed every five years by ISCO3:

• 1st Edition (2010)

• 2nd Edition (2015)

• 3rd Edition (2020)

• 4th Edition (2025)

Its large number of translations into different languages makes it a unique reference document for the scientific practice of ozone therapy worldwide.

Index – Main Sections

General

• Editions of the Declaration

• Authors

• ISCO3 members that approved the Declaration (April 18, 2025)

• What is ISCO3?

• Main objectives of ISCO3

• Disclaimer

• Why a 4th edition of the Madrid Declaration is necessary

• Conclusions

• Recommendations

• Acknowledgements

HUMAN MEDICINE

Therapeutic ranges for the use of ozone

1. Therapeutic bases

   • Growth of scientific publications on ozone therapy

   • Costs and economic benefits

   • Ozone therapy as a complementary technique

   • Ozone therapist

   • Medical ozone generators (MOG)

   • Dose–effect relationship and therapeutic window

   • Materials used in ozone therapy

   • Mechanism of ozone action and redox bioregulation

   • Nutrient supplementation before systemic ozone therapy

   • Most common routes of local application

2. Ozone therapy basic principles (O₃x)

   • Contraindications, warnings and interactions

   • Synergistic effects with other oxidative therapies

   • Adverse effects and toxicity

   • Pediatric dosage (rectal insufflation)

3. Main systemic application routes

   • Major and minor autohemotherapy (MAH, MiAH)

   • Ozonated saline solution (O₃SS): preparation, properties, doses, indications and adverse effects

   • Extracorporeal blood oxygenation (EBOO)

   • Rectal and vaginal insufflation

4. Main local application routes

   • Intramuscular, paravertebral and facet infiltration

   • Hernia, intradiscal, sacral hiatus, intra-articular (knee, hip, etc.)

   • Subcutaneous emphysema (glove technique) and bagging

5. Applications in Dermatology

6. Applications in Aesthetics

7. Vesicourethral lavage

8. Prostate treatment

9. Otic route

10. Ophthalmologic applications

11. Intratonsillar infiltration

12. Microdosing on trigger and acupuncture points

13. Topical applications of ozonized water, saunas, ozonized oils and creams

14. Intraosseous ozone infiltration (IOO₃T)

15. Application routes not recommended because they are unsafe

16. Routes that must not be used under any circumstances

17. Routes that have not received consensus

18. Pathologies most appropriate to be treated with ozone

19. General bases for ozone treatment

ADDENDUM A – APPLICATIONS OF OZONE IN DENTISTRY

Therapeutic ranges for the use of ozone in Dentistry

• Introduction and basic principles

• Safety precautions

• Ozone, ozonized water and oils

• Ozone-compatible materials

• Ozonized bidistilled water

• Disinfection kinetics and ozone ranges

• Microbubbling ozonized saline solution in dentistry

• Evidence-based classification of applications

• Clinical indications: caries, hypersensitivity, periodontal conditions

• Orthodontics

• Oral surgery: extractions, implants, mucositis, PRP activated with ozone

• Crowns, bridges, veneers

• Soft tissue injuries

• Dental whitening with ozone

• Maxillary osteonecrosis

• Temporomandibular disorders (TMD): indications, protocols and recommended approach

• Registration, documentation and biosecurity (dental unit water lines)

ADDENDUM B – APPLICATIONS OF OZONE IN VETERINARY MEDICINE

Therapeutic ranges and clinical applications (small and large animals)

• Introduction

• Main systemic application routes (MAH, MiAH, rectal insufflation, O₃SS)

• Local routes: subcutaneous, intralesional, bagging, vesicourethral-vaginal, intra-articular, paravertebral, trigger points, otic route, ozonized oil

• Administration routes that must not be used

• Routes without consensus and not recommended

• Most common routes and pathologies treated in veterinary medicine

• Ozone therapy in equines and cattle: systemic and local routes, clinical indications

• Tables and clinical guides for small and large animals

Important note:
This product provides digital access to the Madrid Declaration on Ozone Therapy, 4th edition (2025), in English only.
The document is for viewing only and cannot be copied, printed, or downloaded.